| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
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| Autores: |
Sotero-Martins,Adriana
Bon,Elba Pinto da Silva
Carvajal,Elvira
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| Data: |
2003-12-01
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| Ano: |
2003
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| Palavras-chave: |
Asparaginase II
Nitrogen regulation
Saccharomyces cerevisiae
Genomic integration
Green fluorescent protein
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| Resumo: |
Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822003000400017
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.34 n.4 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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